核糖核酸酶Ⅲ(RNASEN)重组蛋白

YB163Hu012
Recombinant Ribonuclease III, Nuclear (RNASEN)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
核糖核酸酶Ⅲ(RNASEN)重组蛋白[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Ile1114~Glu1235
Tags: N-terminal His-Tag
Subcellular Location: Nucleus, nucleolus. Purity: >98%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;
Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
P核糖核酸酶Ⅲ(RNASEN)重组蛋白redicted isoelectric point: 6.9

Predicted Molecular Mass: 15.2kDa
Accurate Molecular Mass: 15kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0
mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
核糖核酸酶Ⅲ(RNASEN)重组蛋白oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. SDS-PAGE